Differences in the methylation of transfer ribonucleic acid in vitro by the mitochondrial and cytoplasmic transfer ribonucleic acid methylases of HeLa cells.

Differences in the Methylation of Transfer Ribonucleic Acid in VVitro by the Mitochondrial and Cytoplasmic Transfer Ribonucleic Acid Methylases of HeLa Cells

The methylation of Escherichia coli B transfer RNA in vitro by HeLa mitochondrial and cytoplasmic extracts was examined. The following differences were found. (a) The cytoplasmic extract methylated E. coli B tRNA to a much greater extent than did the mitochondrial extract. (b) The two extracts methylated different sites on E. coli B tRNA, thereby generating different methylated oligonucleotide ...

Differences in the Methylation of Transfer Ribonucleic Acid in VVitro by the Mitochondrial and Cytoplasmic Transfer Ribonucleic Acid Methylases of HeLa Cells

The methylation of Escherichia coli B transfer RNA in vitro by HeLa mitochondrial and cytoplasmic extracts was examined. The following differences were found. (a) The cytoplasmic extract methylated E. coli B tRNA to a much greater extent than did the mitochondrial extract. (b) The two extracts methylated different sites on E. coli B tRNA, thereby generating different methylated oligonucleotide ...

The postnatal methylation of transfer ribonucleic acid in brain. Evidence for the methylation of precursor transfer ribonucleic acid.

Incubation of 3-day-old rat brain with L-[methyl-3H]methionine resulted in the rapid labeling of low-molecular-weight cytoplasmic RNA. Electrophoresis in 15% polyacrylamide gels provided evidence for the methylation of precursor tRNA molecules, and high-performance liquid chromatography demonstrated N2-methylguanine to be the predominant methylated base formed during the first 2 min of labelling.

Indirect evidence for the presence of Ribonucleic acid in vesicular stomatitis Virions

Inhibitor of transfer ribonucleic acid methylase in the differentiating slime mold Dictyostelium discoideum.

Eight hours after the onset of morphogenesis, an inhibitor of transfer ribonucleic acid methylases appears in differentiating Dictyostelium discoideum. The inhibitor is also present in spores. Fifty per cent of the inhibiting activity is lost upon heating at 100 C for 5 min; it is nondialyzable and sensitive to trypsin.